Discovery of chemically induced mutations in rice by TILLING
نویسندگان
چکیده
منابع مشابه
Discovery of rare mutations in populations: TILLING by sequencing.
Discovery of rare mutations in populations requires methods, such as TILLING (for Targeting Induced Local Lesions in Genomes), for processing and analyzing many individuals in parallel. Previous TILLING protocols employed enzymatic or physical discrimination of heteroduplexed from homoduplexed target DNA. Using mutant populations of rice (Oryza sativa) and wheat (Triticum durum), we developed a...
متن کاملLarge-scale discovery of induced point mutations with high-throughput TILLING.
TILLING (Targeting Induced Local Lesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and low-cost discovery of induced point mutations in populations of chemically mutagenized individuals. As chemical mutagenesis is widely applicable and mutation detection for TILLING ...
متن کاملBreakthrough Technologies Discovery of Rare Mutations in Populations: TILLING by Sequencing
Department of Plant Biology and Genome Center (H.T., T.H., B.W., K.J.N., M.L., R.K.T., A.A.K., L.C.), Department of Plant Sciences (R.N., C.U., T.H.T., J.D.), Department of Computer Sciences (V.M., V.F.), Bioinformatics Core, Genome Center (J.F.), and United States Department of Agriculture Agricultural Research Service, Crops Pathology and Genetics Research Unit (T.H.T.), University of Califor...
متن کاملTILLING and ecotilling for rice.
Mutagenesis is frequently used to test gene function and to aid in crop improvement. Targeting Induced Local Lesions in Genomes (TILLING) is a reverse genetic strategy first developed to identify induced point mutations in Arabidopsis. This general strategy has since been applied to many plant and animal species. Here, we describe a protocol for high-throughput TILLING in rice. Gene segments ar...
متن کاملReverse genetics in zebrafish by TILLING.
TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Dro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Plant Biology
سال: 2007
ISSN: 1471-2229
DOI: 10.1186/1471-2229-7-19